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羽扇豆蛋白的提取及性质研究

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采用碱溶酸沉法对羽扇豆蛋白进行提取,利用响应面法对羽扇豆蛋白的提取工艺进行优化,得到最佳提取工艺是:pH为9.9,液料比15.7,温度为55.5℃,提取时间为30.4min,蛋白最佳得率为13.2%。按照Osborne蛋白分级提取方法对羽扇豆蛋白进行了精细分类,分别得到了羽扇豆清蛋白、球蛋白、醇溶蛋白和谷蛋白,其中球蛋白含量最高。羽扇豆蛋白的功能性质,包括溶解性、持水持油性、乳化性质,也进行了测定。
Extraction and functional propeties of lupin protein
The protein from liupin was extracted by alkali-solution and acid-isolation and the optimal conditions are established as follows:pH 9.9,the ratio of solvent to meal 15.7,temperature 55.5 ℃ and 30.4 min.Under these conditions,the extraction yield was 13.13%.Four fractions of lupin proteins were separated by Osborne method,among which the protein content of albumin was the highest.Some functional properties of the protein,including the solubility,water-and oil-holding capacities,and emulsifying activity,were deter-mined.

lupin proteinextractionOsborne fractionfunctional properties

王姝、王昌涛、于国萍

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东北农业大学食品学院,哈尔滨150030

北京工商大学北京市植物资源研究开发重点实验室,北京100048

羽扇豆蛋白 提取 Osborne分级 功能性质

2012

食品科技
北京市粮食科学研究所

食品科技

北大核心
影响因子:0.622
ISSN:1005-9989
年,卷(期):2012.(5)
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