A method for simultaneous determination of 2 functional factors（D-Saccharic acid 1,4 lactone and D-Saccharic acid）in Kombucha by reverse phase high performance liquid chromatography was developed. Agilent SB-Aq column（4.6 mm×250 mm, 5 μm） was used at 30 % and mobile phase was 0.025 mol/L KH2PO4（pH2.5） with flow rate of 0.5 mL/min. Detection wavelength was 210 nm. Sample size was 10 μL. The relative standard deviations were lower than 3%, and the recoveries were 99.7% to 102.3%.
NETL
NSTL
万方数据
维普
