Ultrasound Extraction and Antioxidant Activity of Total Flavonoids from'Shatangju'Mandarin Drop Fruit
Ultrasonic-assisted extraction of total flavonoids from'Shatangju'mandarin drop fruit was carried out,and its antioxidant activity was studied.On the basis of single factor experiment of ethanol concentration,solid-liquid ratio,ultrasonic time and ultrasonic temperature,the extraction process was optimized by orthogonal test.The antioxidant activity of total flavonoids was evaluated by DPPH free radical,hydroxyl radical scavenging rate and total reducing power.The results showed that the optimal process of ultrasonic extraction of total flavonoids from'Shatangju'mandarin drop fruit was ethanol concentration 50%,solid-liquid ratio 1∶50 g/mL,ultrasonic time 60 min,ultrasonic temperature 70℃Under this condition,the extraction rate of total flavonoids reached 2.61%.At this time,the highest scavenging rate of total flavonoids on DPPH free radical and hydroxyl free radical was 88.89%and 80.77%,which had strong antioxidant capacity.It provided theoretical support for further research and development of total flavonoids from'Shatangju'mandarin drop fruit.
drop fruit of'Shatangju'mandarinultrasound extractionantioxidationtotal flavonoids