Optimization of Extraction Technology for Okra Polysaccharide and Its Anti-Fatigue Effect on Exhausted Mice
The aim was to explore the relieving effect of okra polysaccharides(OP)on exhaustion running fatigue in mice.The extraction process of OP was optimized using response surface methodology,and of its effects on fatigue related indicators in mice was evaluated through exhaustion running tests.The results showed that the optimized extraction process conditions for OP were extraction time of 1 hour,extraction temperature of 49 ℃,and solid-liquid ratio of 1∶18(g/mL).Under these conditions,the extraction rate of OP was(28.95±2.65)％.The average exhaustion running time of each dose group of OP was significantly increased compared to the blank control group(P＜0.05).At the same time,it had a significant increase in muscle glycogen and liver glycogen content,lactate dehydrogenase,superoxide dismutase,catalase,and glutathione peroxidase activities in mice after exercise(P＜0.05),and a significant decrease in creatine kinase activity and malondialdehyde content(P＜0.05).The high-dose group and taurine control group had similar anti fatigue effects on exhausted running mice.The OP have good anti-fatigue effect.
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