Optimization of Preparation of Xylo-Oligosaccharides from Apple Residue by Enzymatic Hydrolysis
Xylo-oligosaccharide(XOS)was prepared from apple residue by enzymolysis after pretreatment with alkaline hydrogen peroxide(AHP).The effects of different mass fractions of H2O2 on the composition and spatial structure of apple residue were studied.The enzymatic hydrolysis of apple residue after AHP treatment was selected to prepare XOS.With the enzymatic hydrolysis time,enzymatic temperature,enzymatic pH and enzyme addition amount as variable factors and the yield of XOS as index,the optimal conditions of enzymatic hydrolysis were determined by single factor test and response surface test.The results showed that the hemicellulose content of apple residue increased from 12.3％ to 16％ after 3％ AHP pretreatment,and the removal rate of lignin reached 46.6％.In addition,the optimum parameters for the preparation of XOS by enzymatic hydrolysis of apple residue were as follows:enzymatic hydrolysis temperature 55 ℃,enzymatic hydrolysis pH6.0,enzymatic dosage 7000 u/g,enzymatic hydrolysis time 18 h.Under these conditions,the highest XOS yield was 27.08％.The experimental conditions for preparing xylo-oligosaccharide by enzymolysis of apple residue have practical value and provide theoretical basis for increasing the added value of apple residue.
apple residuealkaline hydrogen peroxidexylo-oligosaccharideenzymatic hydrolysis process
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