酶解苹果渣制备低聚木糖的工艺优化
Optimization of Preparation of Xylo-Oligosaccharides from Apple Residue by Enzymatic Hydrolysis
戴沙 1陈雪峰 2蔡露阳 1陈梦音 1李艾莲1
作者信息
- 1. 陕西科技大学食品科学与工程学院,陕西西安 710021
- 2. 陕西科技大学食品科学与工程学院,陕西西安 710021;陕西农产品加工技术研究院,陕西西安 710021
- 折叠
摘要
以苹果渣为原料,利用碱性过氧化氢(Alkaline hydrogen peroxide,AHP)预处理后的苹果渣酶解制备低聚木糖(Xylo-oligosaccharide,XOS).研究不同质量分数H2O2对苹果渣组成成分成及空间结构的影响,选取AHP处理后的苹果渣酶解制备XOS,以酶解时间、酶解温度、酶解pH值和酶添加量为变量因子,XOS得率为指标,进行单因素试验和响应面试验确定酶解工艺的最佳条件.结果表明:经3％的AHP预处理后苹果渣半纤维素含量由12.3％上升到16％,木质素的去除率达46.6％;此外,酶解苹果渣制备XOS的最佳工艺参数为:酶解温度55 ℃、酶解pH6.0、酶添加量7000u/g、酶解时间18h,在此酶解条件下,酶解液中XOS得率最高为27.08％.试验所得的酶解处理苹果渣制备低聚木糖工艺条件具有实用价值,可为提高苹果渣附加值提供理论依据.
Abstract
Xylo-oligosaccharide(XOS)was prepared from apple residue by enzymolysis after pretreatment with alkaline hydrogen peroxide(AHP).The effects of different mass fractions of H2O2 on the composition and spatial structure of apple residue were studied.The enzymatic hydrolysis of apple residue after AHP treatment was selected to prepare XOS.With the enzymatic hydrolysis time,enzymatic temperature,enzymatic pH and enzyme addition amount as variable factors and the yield of XOS as index,the optimal conditions of enzymatic hydrolysis were determined by single factor test and response surface test.The results showed that the hemicellulose content of apple residue increased from 12.3％ to 16％ after 3％ AHP pretreatment,and the removal rate of lignin reached 46.6％.In addition,the optimum parameters for the preparation of XOS by enzymatic hydrolysis of apple residue were as follows:enzymatic hydrolysis temperature 55 ℃,enzymatic hydrolysis pH6.0,enzymatic dosage 7000 u/g,enzymatic hydrolysis time 18 h.Under these conditions,the highest XOS yield was 27.08％.The experimental conditions for preparing xylo-oligosaccharide by enzymolysis of apple residue have practical value and provide theoretical basis for increasing the added value of apple residue.
关键词
苹果渣/碱性过氧化氢/低聚木糖/酶解工艺Key words
apple residue/alkaline hydrogen peroxide/xylo-oligosaccharide/enzymatic hydrolysis process引用本文复制引用
基金项目
陕西省农业厅科技创新驱动项目(NYKJ-2022-XA-09)
陕西省科技厅重点研发一般项目(2022NY-14)
陕西省农业厅项目(NYKJ-2021-XA-003)
陕西省未央区项目(202036)
出版年
2024
NETL
NSTL
万方数据