Optimization of Ultrasonic-Assisted Extraction of Total Flavonoids from Leaves of Euryale ferox and the Anti-Lipid Oxidation Ability
The extraction process of total flavonoids from leaves of Euryale ferox by ultrasonic-assisted was explored,single factor test and Box-Behnken(BBD)experimental design were used to optimize the extraction process with the yield of total flavonoids from leaves of Euryale ferox as the response,and the volume fraction of ethanol,solid/liquid ratio,ultrasonic time and ultrasonic temperature were used as independent variables,and the anti-lipid oxidation ability of flavonoids from leaves of Euryale ferox in vitro was analyzed.The results showed that,the optimal extraction process was as follows:68％ for volume fraction of ethanol,1∶19 g/mL for solid/liquid ratio,30 min for ultrasonic time and 49 ℃ for ultrasonic temperature,the yield of total flavonoids was(1.69±0.12)％.Total flavonoids from leaves of Euryale ferox could significantly slow the increasing trend of oil peroxidation value(POV),with a certain amount of concentration-dependent.POV value of animal fat added with 0.05 mg/g total flavonoids from leaves of Euryale ferox was(15.2±1.16)mmol/kg,which was only 33.9％ of the blank control group on the 16th day after high temperature induction,and the POV value of vegetable oil under the same experimental conditions was(32.1±2.15)mmol/kg,which was only 38.6％ of the blank control group,both values were close to the amount of butyl hydroxyanisole(BHA)added 0.03 mg/g.The process conditions were feasible and reliable,the total flavonoids from leaves of Euryale ferox had a certain anti-lipid oxidation ability and provided a theoretical basis for the full development and utilization of local leaves of Euryale ferox resources.
leaves of Euryale feroxtotal flavonoidsultrasound-assisted extractionbutyl hydroxyanisoleanti-lipid oxidation ability
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