首页|辣椒品种对发酵辣椒酱真菌多样性及其理化指标的影响

辣椒品种对发酵辣椒酱真菌多样性及其理化指标的影响

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运用高通量测序技术分析了不同品种辣椒(黄灯笼椒、黄贡椒、红尖椒、青尖椒、指天椒)所制得发酵辣椒酱的真菌多样性,并结合理化指标,探究了辣椒种类对理化特性形成的影响及发酵辣椒酱主要菌属与理化因子之间的相互关系.结果表明,灯笼椒辣椒酱群落多样性和丰富度最高,青尖椒辣椒酱群落多样性和丰富度最低;黄贡椒、青尖椒和指天椒3种辣椒酱的微生物菌群组成具有较高的相似性;Hanseniaspora可能为造成灯笼椒辣椒酱与其他品种辣椒酱差异的主要菌属,Clavispora可能为造成红尖椒辣椒酱与其他品种辣椒酱差异的主要菌属.理化指标表明:青尖椒辣椒酱总酸度显著低于其他4组(P<0.05),其水分含量最高且显著高于其他4组(P<0.05),感官品质较差,故青尖椒不适合制作发酵辣椒酱;另外,发酵辣椒酱中的水分含量越高,unclassified Ascomycota丰度值越高,其优势菌种的丰富度越高.研究结果可为传统发酵辣椒酱的工艺优化和品质改善、新型辣椒酱的研发生产和质量控制提供新思路,同时为适合辣椒酱发酵微生物的筛选提供基础.
Effects of Chili Varieties on Fungal Diversity and Physicochemical Indexes of Fermented Chili Sauce
In this study,the high throughput sequencing technology was used to analyze the fungal diversity of fermented chili sauce with different varieties(Capsicum chinense,Huanggong pepper,Ruber pepper,Capsicum frutescens L.,Capsicum frutescens var),and combined with the physicochemical index,the influence of chili species on the formation of physicochemical characteristics and the correlation between the main bacteria of fermented chili sauce and physicochemical factors were explored.The results showed that the community diversity and richness of chili sauce were the highest in Capsicum chinense,while the community diversity and richness of chili sauce in Capsicum frutescens L.were the lowest;The microflora composition of Huanggong Pepper,Capsicum frutescens L.and Capsicum frutescens var were similar to each other;Hanseniaspora may be the main bacterium responsible for the difference between Capsicum chinense chili sauce and other varieties of chili sauce;Clavispora may be the main bacterium that causes the difference between Ruber pepper chili sauce and other chili sauce varieties.Physical and chemical indexes showed that the total acidity of Capsicum frutescens L.sauce was significantly lower than that of the other 4 groups(P<0.05),and its water content was the highest and significantly higher than that of the other 4 groups(P<0.05),and the poor sensory quality,so Capsicum frutescens L.was not suitable for fermented chili sauce.In addition,the higher the moisture content in fermented chili sauce,the higher the abundance value of unclassified Ascomycota,and the higher the abundance of its dominant strains.The research results provide new ideas for the process optimization and quality improvement of traditional fermented chili sauce,the research and development of new chili sauce production and quality control.At the same time,it provides the basis for screening microorganisms suitable for chili sauce fermentation.

high-throughput sequencingfungal diversityfermented chili saucecorrelation analysischili species

张睿艺、谢辽辽、田星

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湖南中医药大学药学院,湖南长沙 410208

湖南省中医药民族医药国际联合实验室,湖南长沙 410208

高通量测序 真菌多样性 发酵辣椒酱 相关性分析 辣椒品种

湖南省自然科学基金面上项目湖南省教育厅优秀青年项目湖南中医药大学校级科研基金

2023JJ3044522B03782021XJJJ012

2024

食品科技
北京市粮食科学研究所

食品科技

CSTPCD北大核心
影响因子:0.622
ISSN:1005-9989
年,卷(期):2024.49(1)
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