Characteristics of in Vitro Digestion and Fermentation of Kiwi Fruit Powder and Its Effects on Intestinal Flora
The biological activity of kiwi fruit powder was explored to provide a broader prospect and direction for the deep processing of kiwi fruit.This study was also aimed to accelerate the industrialization of kiwi fruit powder.In vitro gastrointestinal digestion and in vitro fermentation test were carried out to compared the changes of bioactive substances in different treatment groups.OUT cluster analysis of intestinal microbiota,relative abundance of flora at phyla and genus levels,and microbial diversity analysis were used to study the in vitro digestion and glycolysis characteristics of kiwi fruit powder and its effects on intestinal microflora.The biological activity and digestive and glycolysis characteristics of kiwi fruit powder were conducted an in-depth study.The result showed that the contents of total sugar and reducing sugar of kiwi fruit powder hardly changed after gastrointestinal digestion in vitro,but decreased significantly after fermentation in vitro,which indicated that the digestion and absorption of total sugar and reducing sugar mainly occurred in the stage of fermentation in vitro.The content of total phenol increased in gastrointestinal digestion stage,but did not change significantly in vitro fermentation stage.Kiwi fruit powder promoted and regulated the production of short-chain fatty acids,which had the effect of improving human intestinal health.After 48 h in vitro fermentation,the acetic acid content of kiwi fruit powder group could reach(1.976±0.112)mg/mL,the propionic acid content could reach(1.964±0.092)mg/mL,and the butyric acid content could reach(0.346±0.012)mg/mL,which was significantly higher than that of control check group and positive control group.Compared with control check group,the relative abundance of Actinomycetes and Bifidobacteria in fruit powder group increased,reaching 34.46％and 33.71％respectively.In kiwi fruit powder group,microbial colony abundance decreased,diversity and uniformity did not change significantly,and community composition was significantly different from that in control check group,which indicated kiwi fruit powder had the effect of regulating intestinal microbial composition and maintaining the stability of intestinal flora,which was a potential prebiotic for improving human health.The study provided theoretical basis for clarifying the biological activity and digestive and glycolysis characteristics of kiwi fruit powder.
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