Effects of Extraction Methods on the Extraction Rate and Physicochemical Properties of Angelica sinensis Polysaccharides
Objective:To study the effects of different extraction methods on the extraction rate and physicochemical properties of Angelica sinensis polysaccharides(ASP).Methods:Using Angelica sinensis as raw material,hot water extraction method,hot water coenzyme method,ultrasonic extraction method,and ultrasonic coenzyme method were used for treatment,the ASP were prepared by concentration,ethanol precipitation,and freeze-drying.The content of the polysaccharides were determined,and their rheological properties were investigated,the three-dimensional helical structure was identified,the thermal stability was analyzed,and the scavenging activity against 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical was evaluated.Results:The ultrasonic coenzyme extraction method had the highest extraction rate of ASP,which was 1.70％.Dynamic rheological analysis showed that the presence of enzymes and high frequency in the extraction method could enhance the gel property of ASP aqueous solution.In addition,four different extraction methods could obtain ASP with high purity,no triple helix structure,and good thermal stability.The highest clearance rate of DPPH radicals was in the range of 92.21％to 94.77％,and the half clearance rate(IC50)was in the range of 1.50 mg/mL to 1.63 mg/mL,with small differences.Conclusion:Ultrasonic coenzyme extraction has the advantages of high extraction rate,easy operation,low cost,and better preservation of the physicochemical properties of ASP,so it can be used as a potential method for extracting ASP.
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