Effect of Chemical Modification on Anti-Tumor Activity of Haematococcus pluvialis Polysaccharide in Vitro
In order to explore the effect of chemical modification on the antitumor activity of Haematococcus pluvialis polysaccharide(HPP)in vitro,HPP was extracted from Haematococcus pluvialis and modified by carboxymethylation,sulfation and acetylation,respectively.The antitumor activity of the modified polysaccharide in vitro was studied.C-HPP was obtained by carboxymethylation of HPP with a degree of substitution of 0.17.HPP was sulfated to obtain S-HPP with a degree of substitution of 0.22.HPP was acetylated to obtain A-HPP with a degree of substitution of 0.25.The results of anti-tumor test showed that C-HPP,S-HPP and A-HPP were superior to HPP in inhibiting the proliferation of HepG-2 and Hela in vitro.Among them,acetylation modification had the best inhibitory effect on the proliferation of the two cells,with IC50 values of 0.637 mg/mL and 0.867 mg/mL,respectively.In summary,the chemical modification of HPP can improve its activity of inhibiting the proliferation of hepatoma cell HepG-2 and cervical cancer cell Hela in vitro.The results provide a basis for the further development and utilization of HPP.
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