Optimization of Extraction Process and Antioxidant Activity of Polyphenol from Chinese Leek
In order to explore the optimal extraction process and in vitro antioxidant activity of Chinese leek polyphenols,this study optimized the ultrasound assisted extraction conditions of Chinese leek polyphenols using response surface methodology,and preliminarily explored the in vitro antioxidant capacity of Chinese leek polyphenol.The optimal extraction process conditions were as follows:ethanol volume fraction of 68％,liquid-material ratio of 31:1,ultrasound power of 79 W,and extraction temperature of 40 ℃,which the polyphenols extraction was 8.69 mg/100 g.An ultra-performance liquid chromatography-mass spectrometry method was used to determine the presence of 16 components in Chinese leeks polyphenols,including two flavonoids,two alkaloids,one organic acid,three terpenoids,three common phenolic compounds and five other compounds.The polyphenols exhibited better scavenging ability for 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)ammonium salt(ABTS)radical cation,hydroxyl radicals and 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals with IC50 values of 131.06,98.61,46.35 μg/mL,respectively.The polyphenols from Chinese leeks had substantial effect on antioxidant activity.
Chinese leekpolyphenolsresponse surface methodologyantioxidant activity
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