Optimization of Extraction and Antioxidant Activities of Chlorogenic Acid from Honey Peach Based on Box-Behnken Response Surface Methodology
The conditions for ultrasonic-assisted aqueous two-phase systems extraction technique of chlorogenic acid from honey peach pulp and kernel,were optimized by Box-Behnken response surface design method.The factors including polyethylene glycol molecular weight,salt type,ultrasonic time,ultrasonic power and temperature were identified as main variables that affect extraction yield.Besides,the antioxidant activities of the extracted chlorogenic acid were studied.The results showed that the optimal extraction parameters from pulp and kernel were as follows:polyethylene glycol molecular weight 600,inorganic salt(NH4)2SO4,ultrasonic temperature 40 ℃,ultrasonic time 32.5,32.0 min,ultrasonic power 300,290 W,respectively.Under these condition,the extraction amount of chlorogenic acid were 123.05 mg/kg FW and 91.83 mg/kg FW.The antioxidant results showed that the scavenging rates of 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical,hydroxyl radical,superoxide anion radical and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)radical cation were 68.63％and 64.75％,63.18％and 56.34％,70.65％and 63.98％,68.19％and 59.98％,respectively.The semi-inhibitory concentration(IC50)showed that the antioxidant activity of chlorogenic acid extract on DPPH radical was better than the other three free radicals,which were 130.19 μg/mL and 135.46 μg/mL.The results of this study indicated that the approach of ultrasonic-assisted aqueous two-phase systems can effectively extract the chlorogenic acid in honey peach,the chlorogenic acid had good antioxidant activity and has potential in-depth development and application value.
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