Extraction of Phycocyanin from Nostoc commune by Aqueous Two-Phase System
The single factor experiment and orthogonal experiment were used to optimize the technology of aqueous two-phase extraction and purification of phycocyanin from Nostoc commune.The effects of PEG1000 content,ammonium sulfate content and ionic strength on the purification of phycocyanin from Nostoc commune were studied in PEG1000/ammonium sulfate system,The recovery rate was used as the test index to determine the optimal conditions of aqueous two-phase extraction and purification of phycocyanin from Nostoc commune.The results showed that the effects of three factors on the recovery rate of phycocyanin from Nostoc commune were in the order of ammonium sulfate＞potassium chloride＞PEG1000 in an orthogonal test.The optimal combination was obtained:with the total mass of 20 g aqueous two-phase system,join 5 g of phycocyanin crude extract,with PEG1000 mass fraction set at 14％,ammonium sulfate mass fraction at 15％,and potassium chloride mass fraction at 0.5％,the purity of phycocyanin was 0.302,and the recovery rate was 96.43％.
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