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高效液相色谱-串联质谱法测定饮料中7种绿原酸类物质

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目的:建立了一种高效液相色谱-串联质谱法(High performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)同时快速、准确测定饮料中7种绿原酸类物质含量的方法.方法:研究不同柱温、流速、色谱柱、提取溶剂、超声时间等对7种绿原酸类物质测定的影响,最终确定试样经90％甲醇水溶液超声提取,采用RP18(2.1 mm×100 mm,1.7 μm)色谱柱分离,HPLC-MS/MS同时测定新绿原酸、绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、1,5-二咖啡酰奎宁酸成分含量.结果:对HPLC-MS/MS检测7种成分的检测条件优化和方法学考察,包括色谱条件.结论:该检测方法简便、准确、可靠,线性关系、精密度、重复性、稳定性、加样回收率均符合要求,可用于饮料中7种绿原酸类成分的定量分析.
Determination of Seven Chlorogenic Acids in Beverages by High Performance Liquid Chromatography-Tandem Mass Spectrometry
Objective:To establish a high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for simultaneous,rapid and accurate determination of seven chlorogenic acids in beverages.Methods:The influence of different column temperature,flow rate,chromatographic column,extraction solvent and ultrasonic time on the determination of seven chlorogenic acids was studied,the samples were extracted ultrasonic by 90％methanol aqueous solution and separated by RP18(2.1 mm×100 mm,1.7 μm)chromatographic column.The contents of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A,isochlorogenic acid B,isochlorogenic acid C and 1,5-dicaffeoyl quinic acid were determined simultaneously by HPLC-MS/MS.Results:HPLC-MS/MS was used to determine the conditions and methods of seven components,including chromatographic conditions.Conclusion:The method is simple,accurate and reliable,the linear relationship,precision,repeatability,stability and recovery rate meet the requirements,it can be used for the quantitative analysis of seven chlorogenic acids in beverages.

beveragehigh performance liquid chromatography-tandem mass spectrometryneochlorogenic acidchlorogenic acidcryptochlorogenic acidisochlorogenic acid Aisochlorogenic acid Bisochlorogenic acid C1,5-dicaffeoyl quinic acid

许佩勤、陈茹、利通、许雪儿

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饮料 高效液相色谱-串联质谱 新绿原酸 绿原酸 隐绿原酸 异绿原酸A 异绿原酸B 异绿原酸C 1,5-二咖啡酰奎宁酸

2024

食品科技
北京市粮食科学研究所

食品科技

CSTPCD北大核心
影响因子：0.622
ISSN：1005-9989
年,卷(期)：2024.49(2)
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