Simultaneous Determination of Deoxynivalenol and Zearalenone in Wheat by High Performance Liquid Chromatography
The method for simultaneous determination of deoxynivalenol(Don)and zearalenone(Zen)in wheat using a two in one immunoaffinity column purification and high performance liquid chromatography with UV detector and fluorescence detector in series was established.The samples were extracted with 60％acetonitrile water,followed by vortexing,shaking,centrifugation,filteration,and the filtrate was purified using a two in one immunoaffinity column.The solution dried by N2 and redissolved by 50％methanol water was detected by a high performance liquid chromatography coupled with a fluorescence detector and an ultraviolet detector.This method can complete the simultaneous detection of two mycotoxins within 25 minutes,and the average correlation of calibration curves for Don and Zen exceeding 0.999.The average recovery rates were 85.8％-95.5％and 87.8％-92.3％％,the relative standard deviations(RSD)were 3.2％-7.8％and 3.2％-7.5％,and the limits of detection were 50 μg/kg and 5 μg/kg,the limits of quantification were 100 μg/kg and 10 μg/kg.This method was sensitive,rapid and exact by the external standard method.
two in one immunoaffinity columnhigh performance liquid chromatographywheatdeoxynivalenolzearalenone
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