Increase the Rate of Pre-enrichment Before Vibrio parahaemolyticus Tested
In order to effectively shorten the pre-enrichment time and broken the technical bottleneck of rapid detection of Vibrio parahaemolyticus,this study explored a technical method to improve the pre-enrichment speed of Vibrio parahaemolyticus by optimizing the culture medium formula and culture conditions.The optimal medium formula obtained through single-factor and response surface analysis:soybean peptone 7.4 g/L,yeast extract 9.8 g/L,and bovine heart extract 20.6 g/L,NaCI 30.0 g/L,pH 8.0.The optimal culture conditions are:temperature 36 ℃,rotational speed 180 r/min.Applying the optimized pre-enrichment culture method,the Vibrio parahaemolyticus culture could be enriched within 6 hours to meet the sensitivity requirements for real-time polymerase chain reaction(PCR)detection.In samples with added food matrices,the optimized pre-enrichment culture method still showed high enrichment efficiency compared to the GB 4789.7.The results indicate that improving culture medium formula and culture conditions can effectively reduce the pre-enrichment time for V.parahaemolyticus detection,Significantly reducing the pre-enrichment time can lay a foundation for achieving rapid detection of foodborne pathogens.
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