提升副溶血性弧菌检验前增菌速度
Increase the Rate of Pre-enrichment Before Vibrio parahaemolyticus Tested
索一平 1胡智恺 1李龙 1李爽 1曹悦 1宋丽萍1
作者信息
- 1. 北京市食品检验研究院(北京市食品安全监控和风险评估中心),国家市场监管重点实验室(食品安全重大综合保障关键技术),北京 100010
- 折叠
摘要
为有效缩短前增菌时间并打破副溶血性弧菌快检的技术瓶颈,研究通过优化培养基配方和培养条件,探索提升副溶血性弧菌前增菌速度的技术方法.通过单因素和响应面分析确定最适培养基配方为:大豆蛋白胨7.4 g/L、酵母浸粉9.8g/L、牛心浸粉20.6 g/L,NaCl 30.0 g/L,pH8.0.最佳培养条件为:温度36 ℃,转速180r/min.应用优化的增菌培养方法,对副溶血性弧菌增菌培养6 h后,即可获得满足荧光聚合酶链式反应(Polymerase chain reaction,PCR)检测灵敏度要求的增菌液.在添加食品基质的样本中,与GB 4789.7增菌方法相比,优化的增菌培养方法表现出了较高的增菌效率.研究结果表明,改良培养基配方和改善培养条件均可以有效缩短副溶血性弧菌检测的前增菌时间.大幅缩短增菌时间可以为实现食源性致病菌快检奠定基础.
Abstract
In order to effectively shorten the pre-enrichment time and broken the technical bottleneck of rapid detection of Vibrio parahaemolyticus,this study explored a technical method to improve the pre-enrichment speed of Vibrio parahaemolyticus by optimizing the culture medium formula and culture conditions.The optimal medium formula obtained through single-factor and response surface analysis:soybean peptone 7.4 g/L,yeast extract 9.8 g/L,and bovine heart extract 20.6 g/L,NaCI 30.0 g/L,pH 8.0.The optimal culture conditions are:temperature 36 ℃,rotational speed 180 r/min.Applying the optimized pre-enrichment culture method,the Vibrio parahaemolyticus culture could be enriched within 6 hours to meet the sensitivity requirements for real-time polymerase chain reaction(PCR)detection.In samples with added food matrices,the optimized pre-enrichment culture method still showed high enrichment efficiency compared to the GB 4789.7.The results indicate that improving culture medium formula and culture conditions can effectively reduce the pre-enrichment time for V.parahaemolyticus detection,Significantly reducing the pre-enrichment time can lay a foundation for achieving rapid detection of foodborne pathogens.
关键词
副溶血性弧菌/前增菌/食源性致病菌/快检Key words
Vibrio parahaemolyticus/pre-enrichment/foodborne pathogenic bacteria/rapid detection引用本文复制引用
基金项目
国家重点研发计划(十四五)(2022YFF1100902)
出版年
2024
NETL
NSTL
万方数据