产α-葡萄糖苷酶抑制剂酵母菌的筛选、发酵条件优化及酶动力学研究
Screening,Fermentation Conditions Optimization and Enzymatic Kinetics of α-Glucosidase Inhibitor-Producing Yeasts
叶茂 1邓毛程 1李静 1宋杨2
作者信息
- 1. 广东轻工职业技术学院食品与生物技术学院,广东省特色调味品工程技术开发中心,广东广州 510300
- 2. 广东轻工职业技术学院食品与生物技术学院,广东省特色调味品工程技术开发中心,广东广州 510300;韩山师范学院生命科学与食品工程学院,广东潮州 521041
- 折叠
摘要
目的:筛选产α-葡萄糖苷酶抑制剂酵母菌,以丰富微生物源α-葡萄糖苷酶抑制剂的来源,开发食用降糖产品.方法:采用4-硝基酚-α-D吡喃葡萄糖苷(p-nitrophenyl-α-D-glucopyranoside,p-NPG)方法从酒糟中筛选产α-葡萄糖苷酶抑制剂能力较强的酵母菌,通过26S rDNA D1/D2区基因序列分析对其进行鉴定,并以α-葡萄糖苷酶抑制率为评价指标,利用单因素试验及正交试验对其进行发酵条件优化.结果:获得1株产α-葡萄糖苷酶抑制剂能力较强的酿酒酵母(Saccharomyces cerevisiae),编号为sg_093,该菌株产α-葡萄糖苷酶抑制剂最佳发酵条件为以3 g/100mL葡萄糖为碳源、1g/100mL酵母抽提物为氮源、初始pH值为7.0、接种量1.5％、温度20 ℃下发酵48 h,所得发酵液的α-葡萄糖苷酶抑制率为66.0％,是优化前的1.5倍,并且是以可逆混合竞争方式抑制α-葡萄糖苷酶.结论:获得1株酿酒酵母,其发酵产物对α-葡萄糖苷酶活性有较好的抑制作用.
Abstract
Objective:Screening the α-glucosidase inhibitor producing yeast,which is rich in microbial sources of α-glucosidase inhibitor,to develop food hypoglycemic products.Methods:Through the screening of yeast in the fermentation process of wine,a strain of yeast with strong capacity of producingα-glucosidase inhibitor was obtained by p-nitrophenyl-α-D-glucopyranoside(pNPG)method,identified by 26S rDNA D1/D2 gene sequence analysis,and the fermentation conditions were optimized by single factor test and orthogonal test,taking the inhibition rate of α-glucosidase as the evaluation index.Results:The Saccharomyces cerevisiae with high yield α-glucosidase inhibitor was obtained and was named sg_093.The optimal fermentation conditions by the strain were 3 g/100 mL glucose as carbon source,1 g/100 mL yeast extract as nitrogen source,initial pH 7.0,inoculum 1.5％,temperature 20 ℃,and time 48 h.Under the optimized conditions,the inhibition rate of α-glucosidase inhibitor reached 66.0％,which was 1.5 times that of before optimization.And inhibiting α-glucosidase in a mixed competitive manner.Conclusion:The strain Saccharomyces cerevisiae was obtained and its fermentation products had a good inhibitory effect on α-glucosidase activity.
关键词
α-葡萄糖苷酶抑制剂/酵母/筛选/发酵条件/优化Key words
α-glucosidase inhibitor/yeast/screening/fermentation condition/optimization引用本文复制引用
基金项目
广东省教育厅特色创新项目(自然科学)(2020KTSCX223)
广东轻工职业技术学院领军拔尖计划(2018)(2B141503)
国家自然科学基金青年基金(31500102)
出版年
2024
NETL
NSTL
万方数据