Preparation and Bactericidal Effect of Hydrogen Peroxide Based Plasma Activated Solution
The purpose of this study was to prepare plasma activated solution(PAS)using H2O2 as the medium,analyze the bactericidal effect on different forms of E.coli and S.aureus,which provided the experimental basis and theoretical guidance for application in the sterilization and preservation of fresh food.The result showed compared with deionized water as the medium,H2O2 significantly(P＜0.05)increased the oxidation reduction potential(ORP)of PAS.Prolonged the plasma treatment time,increased the treatment frequency and the amount of H2O2 in aqueous solution.The optimum preparation process of PAS was as follows:treatment frequency 85 Hz,treatment time 77 s,H2O2 addition 1.0μL/mL.PAS could inhibit different forms of E.coli and S.aureus.Scanning electron microscope(SEM)results showed that PAS could destroy the cell structure of bacteria,thus killing bacteria,and destroy the extracellular polymers in E.coli and S.aureus biofilms.Absorbance of E.coli and S.aureus biofilms decreased significantly(P＜0.05)after PAS treatment of 20 min.
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