Optimization of Preparation Process of Polysaccharide from Agaricus bisporus by Chitosan Flocculation and Assessment of Its Antioxidant Activity
Objective:To optimize the preparation process of polysaccharides from Agaricus bisporus by chitosan-based flocculation and investigate its antioxidant activity.Methods:A comprehensive scoring system was established with analytic hierarchy process(AHP)with polysaccharide recovery rate,protein removal rate and decolorization rate as the indicators,and the orthogonal method was used to optimize the flocculation conditions.The antioxidant activity of ethanol precipitated polysaccharide(E-ABP)and chitosan flocculated polysaccharide(CF-ABP)was measured and E-ABP was taken as the control.Results:The optimum flocculation conditions were as follows:2％flocculant addition,45 ℃,120 min,pH 4.The yield and purity of CF-ABP was(7.64±0.49)％and(60.76±0.70)％,respectively.The IC50 values of the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging capacity of CF-ABP and E-ABP were 1.468 mg/mL and 7.246 mg/mL,respectively.And the IC50 values of the hydroxyl radical scavenging capacity of CF-ABP and E-ABP were 0.122 mg/mL and 0.216 mg/mL,respectively.With a concentration of 4 mg/mL,the total reducing power of CF-ABP and E-ABP were 0.51 and 0.27,respectively.Conclusion:Chitosan flocculation method can be used for the preparation of polysaccharide from Agaricus bisporus,and the CF-ABP shows better antioxidant activity compared to E-ABP.This result provides a new reference for the process of preparation of polysaccharide from Agaricus bisporus.
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