摘要
目的:进一步研究干、鲜马齿苋的降血糖活性及降糖机制.方法:分别将干燥和新鲜马齿苋用乙醇为溶剂浸泡提取,制备干燥马齿苋乙醇提取物(Dried Portulaca oleracea L.ethanol extracts,DEP)和新鲜马齿苋乙醇提取物(Fresh Portulaca oleracea L.ethanol extracts,FEP).再分别将DEP和FEP用水混悬,依次用正己烷、乙酸乙酯、正丁醇进行萃取,从DEP得到H-DEP、E-DEP、D-DEP和W-DEP,从FEP得到H-FEP、E-FEP、D-FEP和W-FEP.分别对DEP和FEP以及所得各种萃取物进行酶抑制活性测试,结果:E-FEP对2种酶的抑制作用最好,IC50值分别为(0.094±0.001)mg/mL和(33.63±0.42)μg/mL.绘制Lineweaver-Burk曲线确定酶抑制类型:对于α-淀粉酶,H-DEP、E-DEP、H-FEP及E-FEP为非竞争性抑制,DEP、FEP、D-DEP、W-DEP、D-FEP及W-FEP为竞争性和非竞争性混合抑制;对于α-葡萄糖苷酶,H-DEP、E-DEP、H-FEP及E-FEP为非竞争性抑制,DEP、FEP、D-DEP、W-DEP、D-FEP及W-FEP为反竞争性和非竞争性混合的抑制类型.结论:马齿苋具有良好的降血糖活性,该研究结果可为综合利用马齿苋资源提供理论依据,对研究开发新型降糖食物、推动降血糖活性研究进展等具有重要意义.
Abstract
Objective:To further investigate the hypoglycemic activity and hypoglycemic mechanism of dried and fresh Portulaca oleracea L.(purslane).Methods:Ethanol extracts were obtained from dried purslane(DEP)and fresh purslane(FEP),after filtration and concentrate.Ethanol extract of dried and fresh purslane were extracted with n-hexane,ethylacetate and n-butanol,the DEP was further separated by different polar solvents and obtained four components:H-DEP,E-DEP,D-DEP,and W-DEP.the FEP was further separated by different polar solvents and obtained four components:H-FEP,E-FEP,D-FEP,and W-DFP.The inhibition activity on α-amylase and α-glucosidase were determined.Results:E-FEP showed the best inhibition effect for both enzymes,with IC50 values of(0.094±0.001)mg/mL and(33.63±0.42)μg/mL.The Lineweaver-Burk curve was plotted to determine the type of enzyme inhibition.The results showed that H-DEP,E-DEP,H-FEP and E-FEP were sensitive to α-amylase,which were the type of noncompetitive inhibition.DEP,FEP,D-DEP,W-DEP,D-FEP and W-FEP were competitive and non-competitive mixed inhibition.For α-glucosidases,the results showed that H-DEP,E-DEP,H-FEP and E-FEP were the type of noncompetitive inhibition.DEP,FEP,D-DEP,W-DEP,D-FEP and W-FEP were anti-competitive and non-competitive mixed inhibition type.Conclusion:Purslane has good hypoglycemic activity.The research results provide a theoretical basis for the comprehensive utilization of purslane resources,and are of great significance for the research and development of hypoglycemic foods and the advancement of hypoglycemic activity research.
基金项目
黑龙江省属高等学校基本科研业务费科研项目(145109512)
NETL
NSTL
万方数据