Optimization of Phosphorylation of Oligosaccharides from Red Chicory(Cichoricum intybus var.foliosum Hegi)by Response Surface Method
Red chicory(Cichoricum intybus var.foliosum Hegi)oligosaccharides were produced from red chicory polysaccharides by trifluoroacetic acid(TFA)degradation method.The phosphorylation of red chicory oligosaccharides was carried out by phosphate method,and the process conditions of phosphorylated red chicory oligosaccharides were optimized by response surface methodology,and the antioxidant activity of phosphorylated red chicory oligosaccharides was investigated.The optimum conditions for the preparation of phosphorylated red chicory oligosaccharide were determined as follows:the molar ratio of red chicory oligosaccharide to phosphorylation reagent was 1∶10,the reaction pH was 7,and the reaction time was 3.2 h at 82.5℃,and the phosphate content of phosphorylated red chicory oligosaccharide could reach 5.48％.The results of infrared spectroscopy confirmed that the modified red chicory oligosaccharides contained phosphate groups.The results of the antioxidant activity test showed that the scavenging rate of hydroxyl radicals by phosphorylated red chicory oligosaccharide increased by 15.87％compared to red chicory oligosaccharide,indicating that the antioxidant activity of phosphorylated modified red chicory oligosaccharide was significantly increased.
red chicory oligosaccharidesphosphorylationresponse surfaceantioxidant activity
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