Comparative Analysis of Different Nucleic Acid Extraction Methods for Detecting Animal-Derived Components in Gelatin
In order to select a suitable method for extracting nucleic acids from edible gelatin,the deoxyribonucleic acid(DNA)from bovine skin gelatin,bovine bone gelatin,pig skin gelatin and fish skin gelatin were extracted by four methods:standard recommended method,improved cetyltrimethyl ammonium bromide(CTAB)method,QIAGEN food genome DNA extraction kit method and TIANGEN deeply processed food DNA extraction kit method.The extraction effects of different methods were compared based on DNA purity,concentration and amplification efficiency of fluorescent polymerase chain reaction(PCR).The results showed that the DNA extracted by the standard recommended method had low purity,severe protein contamination,and low success rate of fluorescent PCR amplification.The DNA extracted by TIANGEN kit method exhibited high purity,but it only demonstrated good detection effect on skin source gelatin,with limited scope of application.The improved CTAB method and QIAGEN kit extracted DNA with high purity,less impurities and little inhibition of fluorescent PCR.The corresponding animal derived was detected in all samples except fish skin gelatin,and can be used for DNA extraction of most gelatin samples.
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