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杨树桑黄液体培养过程中活性成分含量变化规律

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桑黄液体培养与人工栽培相比具有许多优点,并逐步实现工厂化和自动化生产.文章以杨树桑黄为研究对象,通过连续20 d液体培养,测定其菌丝体生物量及菌丝体和发酵液中的多糖含量、三萜含量、黄酮含量、多酚含量等指标,对其进行动态检测与分析.结果表明,杨树桑黄菌丝体生物量和胞外多糖含量在12 d最高;在第8天时,pH值最低,此时发酵液中的黄酮含量、多酚含量最高,三萜含量也处于第一个峰值;菌丝体多糖含量、三萜含量、黄酮含量、多酚含量峰值处于10~12d内.因此,可以根据不同培养时间获得不同次级代谢产物.该研究结果为更好地控制桑黄液体培养过程,以及提高其活性成分含量提供了理论支持.
Activity Component Content Change Rule of Sanghuangporus vaninii During Liquid Culture
The liquid culture of Sanghuangporus spp.has various advantages over artificial cultivation,and is presently advancing towards factory and automated production.In this study,Sanghuangporus vaninii(S.vaninii)was used as the research subject,the mycelium biomass,crude polysaccharide content,triterpene content,flavonoid content,and polyphenol content in both the mycelium and fermentation broth were determined through continuous liquid culture for 20 days,subsequently,dynamic detection and analysis of these indexes were performed.The study found that the biomass and extracellular polysaccharide of S.vaninii mycelium reached their highest levels on day 12.The pH value was lowest on 8th day,coinciding with the highest flavonoid and polyphenol content in the fermentation broth.The triterpene content also reached its first peak on 8th day.The mycelium's polysaccharide,triterpene,flavonoid,and polyphenol contents all peaked between day 10 and day 12.For this reason,various secondary metabolites can be obtained based on varying incubation times.This study's findings offer theoretical support for more effective control of the liquid culture process of S.vaninii and for enhancing the output of its active constituents.

Sanghuangporus vaniniiactive ingredientliquid culturesecondary metabolites

陆源添、常悦、吴悦、刘迪

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延边大学农学院,吉林延吉 133002

延边大学融合学院,吉林延吉 133002

延边大学农学院,食(药)用真菌研究所,吉林延吉 133002

杨树桑黄 活性成分 液体培养 次级代谢产物

国家重点研发计划子课题中韩国际农业技术合作项目延边大学校企合作项目

2017YFD0300104-02延大横向[2019]第19号延大科合字[2020]2号

2024

食品科技
北京市粮食科学研究所

食品科技

CSTPCD北大核心
影响因子:0.622
ISSN:1005-9989
年,卷(期):2024.49(4)