Effect of Aksu-grown Candy Apple on Intestinal Microflora of Mice
In order to investigate the impact of Aksu-grown candy apple on the intestinal microbiota of mice,four groups of 5-week-old male Kunming mice were orally administered daily with candy core of Aksu-grown candy apple,Aksu-grown candy apple flesh(non-candy core),sorbitol,and physiological saline,respectively.After 21 days,the mice's colon segments were dissected under sterile conditions,selective amplification of the colon segments'16S rRNA was performed,and the amplified gene sequences were subjected to high-throughput sequencing using Illumina Novaseq 6000 to analysis the diversity,richness,microbial composition,and functional gene metabolism prediction of the mice's intestinal microbiota was conducted.The results indicated that oral administration of Aksu-grown candy apple did not significantly alter the richness and diversity of the normal mouse intestinal microbiota.However,administration of Aksu-grown candy apple flesh(non-candy core)significantly increased the abundance of Proteobacteria phylum,accompanied by an upregulation of genes controlling metabolism.Administration of Aksu-grown candy apple candy core led to a significant reduction in Bacteroidales class and a notable increase in Bacilli class.Oral administration of sorbitol may to some extent inhibit the growth of Proteobacteria.These findings provide a theoretical basis for further exploring the intrinsic value of Aksu-grown candy apple at a deeper level.
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