Changes in Microbial Community Structure of Grouper During Refrigeration Based on High-Throughput Sequencing
In order to investigate the changes in microbial community structure of grouper during 4 ℃refrigeration,Illumina high-throughput sequencing technology was carried out to analyze the diversity of bacterial,archaea,and fungal communities of grouper during 0-9 days of refrigeration.Alpha diversity index analysis showed that the abundance and diversity of microbial communities in samples during different stages of refrigeration were in the order of bacteria,fungi,and archaea,from high to low.The microbial community structure analysis indicated that group succession was existent in the main dominant microbiota of various microorganisms in grouper during the refrigeration period.During the refrigeration process(3rd to 9th days),the relatively abundance stable dominant bacterial communities included Phenobacterium,unclassified Clostriales ⅩⅢ,unclassified Ruminococcaceae,and unclassified Clostriales,the dominant archaea flora included Metanothrix and Metanobacterium,the dominant fungal communities included Issacchenkia,Rhizomucor,Cutaneotrichosporon,and Discolouides.Principal component analysis showed that there was a smaller difference in the structure of bacteria communities during 3-9 days of refrigeration compared to archaea and fungal.PICRUSt functional prediction of bacterial groups showed that after 3 days of refrigeration,a significantly increment was observed in the relative abundance of genes related to amino acid metabolism,signal transduction,aldol metabolism,drug resistance transport,response regulation,and ion transport.The results can provide theoretical reference for further optimizing the storage and preservation technology of grouper from the perspective of microbial inhibition mechanism.
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