Optimization of Hydrolysis Conditions for Enzymatic Preparation of Arabinoxylo-Oligosaccharides by Psyllium Husk
Psyllium husk contains abundant arabinoxylan,which can be hydrolyzed by xylanase and arabinosidase to prepare arabinoxylo-oligosaccharides.Compared with traditional oligosaccharide production processes,it fully embodies the advantages of green manufacturing.The article uses arabinoxylan in psyllium husk as the substrate,and uses a laboratory produced xylanase and arabinosidase composite enzyme solution.The preparation conditions of arabinoxylo-oligosaccharides are optimized using a combination of single factor optimization and response surface analysis.The single factor optimization results showed that when the enzyme hydrolysis time was 5 days,the concentration of arabinoxylan added was 15 g/L,the amount of xylanase added was 100 IU/g arabinoxylan,the pH value of the hydrolysis solution was 4.8,and the enzyme hydrolysis temperature was 50 ℃,the yield of arabinoxylo-oligosaccharides reached the highest value,which was(23.18±0.87)％.In addition,response surface methodology was used to explore the interaction between the concentration of arabinoxylan,the amount of xylanase added,and the enzyme hydrolysis temperature.Finally,the enzyme hydrolysis conditions were determined:when the concentration of arabinoxylan added was 13.90 g/L,the amount of xylanase added was 101.35 IU/g arabinoxylan,and the enzyme hydrolysis temperature was 49.70 ℃,the yield of arabinoxylo-oligosaccharides reached the optimal value(23.67±0.27)％.Verified experiments confirmed that the model's prediction accuracy was high.
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