Preparation Process Optimization,Structure Identifification and Antioxidant Activity of Xylo-Oligosaccharides from Miscanthus Lutarioriparius
Response surface methodology was used to optimize the preparation process of xylo-oligosaccharides from Miscanthus lutarioriparius,and the structure and antioxidant activity of the prepared xylo-oligosaccharides were identied.The xylo-oligosaccharides were first prepared by enzymatic hydrolysis,followed by Sevage method to remove protein,and then the identification of Miscanthus lutarioriparius xylo-oligosaccharides was carried out by ultraviolet spectrum,scanning electron microscopy,Fourier transform infrared spectroscopy,and high performance liquid chromatography,and the antioxidant activity were determined.The optimum conditions were determined as follows:enzymatic hydrolysis at pH5.1,temperature of 48 ℃,time of 14.5 h,and xylanase addition of 0.38％,under these conditions,the yield of xylo-oligosaccharides was(29.94±0.53)％.The structural identification showed that xylo-oligosaccharides was primarily composed of xylobiose,xylotriose,and xylotetraose,and the main chain was composed of β-D-xylulose linked by β-1,4 glycosidic bonds,and the side chain contained a small amount of glucuronic acid with an irregular pore structure.Antioxidant activity tests showed that xylo-oligosaccharides had IC50 values of 0.817 mg/mL and 2.256 mg/mL for clearing 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)radical cation,respectively,and an IC50 value of 6.135 for ferric ion reduction.This study provides theoretical basis for the development of functional food products based on xylo-oligosaccharides from Miscanthus lutarioriparius.
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