Response Surface Optimization of Extraction Process and Antioxidant Activity of Polysaccharide from Melastoma dodecandrum Lour.
Single factor test and response surface method were used to optimize the ultrasound-complex enzymatic extraction process of Melastoma dodecandrum Lour.polysaccharide(MDP).The composition and structural characteristics of MDP were analyzed by high performance liquid chromatography and infrared spectroscopy,and the antioxidant capacity of MDP was explored by detecting its ability to eliminate 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)radical cation and hydroxyl radicals.The results showed that under the conditions of 1.9％papain,3.0％cellulase,67 ℃,and 70 min,the yield of MDP reached the highest((1.364±0.004)％).Eight monosaccharide components were identified in the polysaccharide,of which galacturonic acid,galactose and arabinose were the main components.MDP exhibited ABTS radical cation eliminating ability(IC50=3.047 mg/mL),DPPH radical eliminating ability(IC50=26.93 μg/mL)and hydroxyl free radical eliminating ability(IC50=7.457 mg/mL),and had strong antioxidant ability.The antioxidant strength showed a dose-dependent manner.This study provides a theoretical basis for the development and application of MDP.
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