Extraction Process Optimization and Probiotics of Houttuynia cordata Extract
In order to optimize the extraction process of Houttuynia cordata extract(HCE)and test the probiotic ability of HCE,the effects of liquid to material ratio,extraction time,and extraction temperature on the extraction rate of HCE were studied through a combination of single factor experiments and response surface methodology.The optimal extraction conditions were obtained as follows:liquid to material ratio of 10∶1,extraction temperature of 55 ℃,and extraction time of 150 minutes,with a maximum extraction rate of 25.68％.When the HCE concentration was 20％,it could significantly improve the proliferation ability of B.longum(P＜0.05).When the HCE concentration was 15％,the acid production ability of B.longum could be significantly improved(P＜0.05);When the HCE concentration was 10％,it could significantly improve the proliferation ability of L.bulgaricus and the acid production ability of B.longum,L.bulgaricus,and L.acidophilus(P＜0.05);When the HCE concentration was 5％,it could significantly improve the proliferation ability and acid production ability of L.acidophilus(P＜0.05).From the above results,it could be concluded that the HCE could significantly promote the proliferation and acid production ability of probiotics.
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