产愈创木酚的假单胞菌在白酒酿造中的应用初探
Enhanced Application of A Strain of Pseudomonas azogenes with High Guaiacol-Producing Capacity in Baijiu-Brewing
邓照 1杜亚珂 1夏玙 1林颖 1熊灵 1五味胜也 2张文学3
作者信息
- 1. 四川大学轻工科学与工程学院,四川成都 610044
- 2. 东北大学农业科学研究生院,日本仙台980-8572
- 3. 四川大学轻工科学与工程学院,四川成都 610044;四川大学锦江学院白酒学院,四川眉山 620860
- 折叠
摘要
为提高白酒中愈创木酚类物质浓度,将一株产愈创木酚的YF-58产氮假单胞菌用于制备细菌麸曲.以愈创木酚浓度为指标,固定培养温度为30 ℃,通过单因素试验确定细菌麸曲的最佳水分含量、接种量和培养时间,并将所得细菌麸曲按照不同添加比例进行实验室条件下酱香型白酒酿造试验.结果表明,细菌麸曲的最佳培养条件为水分含量40％、接种量7％、培养温度30 ℃、培养时间3d,酿酒用曲最佳添加比例是高温大曲:细菌麸曲为3:2,此条件下所酿造白酒的出酒率、愈创木酚浓度和4-乙基愈创木酚浓度较对照组分别提高了39％、223％、130％.该研究可为后续提高白酒中愈创木酚浓度提供一定的理论依据.
Abstract
In order to increase the content of guaiacol in Chinese Baijiu,a strain of Pseudomonas YF-58 with high guaiacol-producing capacity was used to prepare bacterial Fuqu.With the concentration of guaiacol as the index,the optimum water content,inoculation amount and culture time of bacterial Fuqu were determined at 30 ℃.The obtained bacterial Fuqu was tested for solid-state brewing experiments of Moutai-flavored Baijiu according to different addition amounts.The results showed that the optimal conditions for the bacterium Fuqu were 40％moisture,7％inoculation amount,30 ℃ incubation temperature,3 days of incubation time,and the optimal ratio of Fuqu for sake brewing is 3∶2 for high-temperature Daqu:bacterial Fuqu.Under these conditions,the yield,guaiacol concentration and 4-ethyl guaiacol concentration of Baijiu were increased by 39％,223％and 130％,respectively.This study provides a theoretical basis for increasing the concentration of guaiacol in Baijiu.
关键词
愈创木酚/产氮假单胞菌/细菌麸曲/工艺优化Key words
guaiacol/Pseudomonas/bacterial Fuqu/process optimization引用本文复制引用
基金项目
国家重点研发计划项目(2018YFE0127400)
出版年
2024
NETL
NSTL
万方数据