Enhanced Application of A Strain of Pseudomonas azogenes with High Guaiacol-Producing Capacity in Baijiu-Brewing
In order to increase the content of guaiacol in Chinese Baijiu,a strain of Pseudomonas YF-58 with high guaiacol-producing capacity was used to prepare bacterial Fuqu.With the concentration of guaiacol as the index,the optimum water content,inoculation amount and culture time of bacterial Fuqu were determined at 30 ℃.The obtained bacterial Fuqu was tested for solid-state brewing experiments of Moutai-flavored Baijiu according to different addition amounts.The results showed that the optimal conditions for the bacterium Fuqu were 40%moisture,7%inoculation amount,30 ℃ incubation temperature,3 days of incubation time,and the optimal ratio of Fuqu for sake brewing is 3∶2 for high-temperature Daqu:bacterial Fuqu.Under these conditions,the yield,guaiacol concentration and 4-ethyl guaiacol concentration of Baijiu were increased by 39%,223%and 130%,respectively.This study provides a theoretical basis for increasing the concentration of guaiacol in Baijiu.