Study on Fermentation Technology of Extracellular Polysaccharide from Ophiocordyceps sinensis and Its Inhibition of Proliferation of Human Colon Cancer Cells in Vitro
The optimal liquid fermentation process for Ophiocordyceps sinensis extracellular polysaccharides(OPS)fermentation was studied using Box Benhnken combination design and response surface analysis,using Ophiocordyceps sinensis as the fermentation strain.The optimal liquid fermentation process was found to be pH 6.17,glucose 33.36 g/L,and silkworm chrysalis powder 21.43 g/L.Under these conditions,the OPS content could reach 355.79 mg/L,which was consistent with the theoretical value.On this basis,the inhibition rates of OPS on HT-29 cells were measured using a cell proliferation detection kit at concentrations of 100,200,400,600 μg/mL,respectively.It was found that OPS had a good inhibitory effect on HT-29 cells.Flow cytometry analysis showed that the inhibitory effect of OPS on the growth of HT-29 cells was related to OPS induced apoptosis,with an increase in early cell apoptosis rate from 2.34％to 24.0％.After OPS treatment,the number of S-phase cells increased to 37.64％,and the number of G0/G1 phase cells decreased accordingly.OPS caused cell cycle arrest in the S-phase.This research result could provide new ideas for the development and utilization of OPS,and provide theoretical basis for its development and application in food and medicine.
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