Simultaneous Determination of 26 Sex Hormones in Fried Chicken Food by QuEChERS Combined with Parallel Liquid Chromatography-Tandem Mass Spectrometry
A method for the simultaneous determination of 26 sex hormone residues in fried chicken food was developed using parallel liquid chromatography-tandem mass spectrometry(UPLC/UPLC-MS/MS)combined with quick,easy,cheap,effective,rugged,and safe(QuEChERS).The samples were extracted with acetonitrile(waters),cleaned-up by 100 mg of octadecylsilane(C18)and 100 mg of ethylenediamine-N-propylsilane powder,concentrated and reconstituted in 50％methanol,and then analyzed by the gradient elution on a Waters ACQUITY UPLC BEH C18 column,with the mobile phases of methanol-2 mmol/L ammonium acetate containing 0.01％ammonia,and the separation was performed by electrospray ionization in positive-negative ionization switching,and the electrochemical separation with the mobile phases of electrochemistry,electrochemical analysis,and electrophoresis.The determination was carried out in electrospray-positive and negative ion switching,multiple reaction monitoring(MRM)mode,and quantified by matrix-matched standard curve external standard method.The method validation showed that the linearity of the 26 sex hormones was good in the range of 1.0-50.0 μg/L with the correlation coefficients(R2)greater than 0.999,and the limits of detection(LODs)of the method were in the range of 0.3-1.0 μg/kg,and the limits of quantification(LOQs)were in the range of 1.0-3.0 μg/kg.The recoveries of 26 sex hormones ranged from 78.9％to 105.3％with the relative standard deviations(RSDs)of 3.1％to 8.4％.The established method is simple,efficient,accurate,reproducible and suitable for the simultaneous determination of 26 sex hormones in fried chicken food.
Quick,easy,cheap,effective,rugged,and safeparallel liquid chromatography-tandem mass spectrometryfried chickensex hormones
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