Isolation,Identification and Whole-Genome Sequencing of Selenium-Tolerant Priestia megaterium
Selenium-enriched microorganisms play an important role in the selenium ecosystem cycle,and they can directly or indirectly introduce selenium into the food production chain through the agricultural-food system,thereby affecting animal and human health.In this study,a selenium-tolerant bacterium was isolated from animal feces.Species identification was performed through 16S rDNA sequencing and whole-genome sequencing,revealing it to be a strain of Priestia megaterium,named N-5.Selenium tolerance experiments showed that N-5 had a strong tolerance to selenium,with a selenium tolerance capacity of up to 16 mmol/L.Whole-genome sequencing and analysis revealed that the genome size of Priestia megaterium N-5 was 5187490 bp,with a GC content of 45.61％,encoding a total of 5244 genes.The genome sequencing data was submitted to NCBI and assigned the GenBank accession number JAZDWX000000000.Using antiSMASH,six gene clusters related to the biosynthesis of secondary metabolites were predicted in the N-5 genome,including four gene clusters with unknown functions,two highly similar antibiotic synthesis gene clusters(Koranimine and Carotenoid),and two antibiotic gene clusters with similarities of only 23％and 13％(Synechobactin and Surfactin),respectively.Additionally,one bacteriocin-encoding gene cluster was discovered,corresponding to the product Sactipeptides.Through evolutionary genealogy of genes:non-supervised orthologous groups(eggNOG)database annotation,amino acid metabolism and carbohydrate metabolism were found to be the main metabolic functions of this strain.Meanwhile,a total of 16 selenium-related protein genes were predicted in the N-5 genome,which may be closely related to the selenium tolerance mechanism of this strain.These findings provide reference and insights for the development and utilization of selenium-enriched probiotic resources.
万方数据
维普
