Biological Activity of Composite Protease Hydrolyzed Ruditapes philippinarum and Its Products
Protease hydrolysis of clam protein to prepare bioactive peptides can increase the products added value.This study used Ruditapes philippinarum as raw material,and selected composite enzymes to screen the combination of proteases and enzymatic hydrolysis conditions based on hydrolysis degree and antioxidant activity.The results showed that the degree of hydrolysis of clam protein reached 78.17％when papain,bromelain,and neutral protease were combined in a 2∶1∶1 ratio,under the conditions of enzyme addition of 0.1％,substrate concentration of 5％,pH 7,temperature of 40 ℃,and hydrolysis 3 h.After ultrafiltration of the hydrolysate,the fraction with a molecular weight of less than 3 ku had 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging rate of 86.92％,hydroxyl free radical scavenging rate of 89.48％,and total reducing power of 0.50.Composite proteases could significantly improve the hydrolysis degree and biological activity of clam protein and the results provide experimental basis for the deep application clams.
万方数据
维普
