Objective:Extraction process of flavonoids from Dendrobium huoshanense was optimized and its anti-fatigue properties were studied.Method:The single-factor tests and response surface analysis was adopted to optimize the flash extraction process of Dendrobium huoshanense flavonoids.The anti-fatigue capacity was evaluated through a weight-loaded swimming test in mice,measuring indicators such as blood lactic acid,blood urea nitrogen,and hepatic glycogen.Result:The optimized processing conditions were determined to include an extraction voltage 112 V,extraction time 54 s,ethanol concentration 52％and substrate-to-solvent ratio 1∶34(g/mL).Under these conditions,the extraction yield of flavonoids from Dendrobium huoshanense was(1.29±0.0697)mg/g.Animal experiments showed that flavonoids from Dendrobium huoshanense could increase the swimming time of weight-loaded mice,reduce blood lactic acid and blood urea nitrogen levels(P＜0.05),and increase liver glycogen levels(P＞0.05).Conclusion Dendrobium huoshanense flavonoids could improve the anti-fatigue ability on mice,which provided a basis for the further research and development of Dendrobium huoshanense flavonoids.
维普
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