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热处理及均质方式对核桃蛋白稳定Pickering乳液性质的影响

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为提高核桃粕利用率,以核桃粕为原料提取核桃蛋白(Walnut protein,WP),探究不同热处理温度(50、60、70、80、90 ℃)对WP的影响.以热处理核桃蛋白(Heat-treated walnut protein,H-WP)为乳化剂制备Pickering乳液,探究不同均质方式(高速分散、超声波)对乳液性质的影响.结果表明,与WP相比,H-WP热稳定性降低.50 ℃时,WP二级结构中β-折叠结构含量最高,蛋白呈紧密球状结构.以H-WP(50 ℃)为乳化剂,通过高速分散制备的Pickering乳液综合性能最佳,平均粒径为11.47 μm,乳液液滴分布均匀,其表观黏度和储能模量较高,表现出较好的凝胶特性,储藏10 d无分层现象.
Effect of Heat Treatment and Homogenization on Properties of Pickering Emulsion Stabilized by Walnut Protein
In order to improve the utilization of walnut meal,this study extracted walnut protein(WP)from walnut meal as raw material and investigated the effects of different heat treatment temperatures(50,60,70,80,90 ℃)on WP.Pickering emulsions were prepared using WP and heat-treated Walnut protein(H-WP)as emulsifiers,and investigated the effects of different homogenization methods(high-speed dispersion,ultrasonic)on the properties of emulsions.The results showed that the thermal stability of H-WP was reduced compared to WP,and the relative content of β-fold in the secondary structure of WP was the highest at 50 ℃,and the protein was in a compact globular structure.Pickering emulsion prepared with high speed dispersion using H-WP at 50℃ as the emulsifier had the best overall performance,with the average particle size of 11.47 μm,the emulsion droplets were uniformly distributed,and its apparent viscosity and storage modulus were higher and its apparent viscosity and energy storage modulus were high,and it showed better gel characteristics,and showing no delamination phenomenon after 10 d of storage.

walnut proteinheat treatmentPickering emulsionhomogenization methodstability

邓艳梅、毕会敏、范方宇

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西南林业大学生命科学学院,云南昆明 650224

西南地区生物多样性保育国家林业和草原局重点实验室,云南昆明 650224

云南省森林灾害预警与控制实验室,云南昆明 650224

核桃蛋白 热处理 Pickering乳液 均质方式 稳定性

云南省兴滇英才支持计划青年人才专项国家自然科学基金项目

YNWR-QNBJ-2018-04631760470

2024

食品科技
北京市粮食科学研究所

食品科技

CSTPCD北大核心
影响因子:0.622
ISSN:1005-9989
年,卷(期):2024.49(6)
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