In order to investigate the inhibitory effect of carvacrol on Listeria monocytogenes,the inhibitory effect of carvacrol on Listeria monocytogenes was determined by adding different concentrations of carvacrol,providing a theoretical basis for its antimicrobial preservation application in chilled meat.The results showed that when the concentration of carvacrol was greater than or equal to 0.20 μL/mL,it significantly inhibited the swimming,swarming,extracellular protease production and biofilm capacity of Listeria monocytogenes.Treatment with 0.10-0.30 μL/mL of carvacrol,the biofilm of Listeria monocytogenes was reduced by 34.73％-74.82％compared to the blank group(P＜0.05).The results of nucleic acid leakage showed that carvacrol treatment caused damage to the cell membrane of Listeria monocytogenes,resulting in leakage of nucleic acid and other contents of cell.In conclusion,carvacrol exhibited significant inhibitory effects on Listeria monocytogenes by disrupting the cell membrane and inhibiting biofilm formation.
维普
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