A method for the determination of 3-acetyl-2,5-dimethylfuran in food by gas chromatography-mass spectrometry was established.Samples were extracted by ultrasound with n-heptane,and the extraction liquid was frozen to remove fat after centrifugation,and then the supematant was purified using dispersive solid-phase extraction.Finally,the purified liquid was analyzed by gas chromatography-mass spectrometry in the selected ion monitoring(SIM)mode.For quantification of 3-acetyl-2,5-dimethylfuran,the external standard method was utilized.The results showed that the method had a good linearity(r＞0.999)within the concentration range of 0.005-0.250 μg/mL for 3-acetyl-2,5-dimethylfuran,and the limits of detection and quantitation were determined to be 0.010 mg/kg and 0.030 mg/kg,respectively.The average recoveries of four food substrates were 93.1％-106.0％in the three levels of spiked food matrices with relative standard deviations(RSD,n=6)ranging from 0.74％to 3.47％.The results showed that the method was simple,sensitive and accurate,which could be used for the determination of 3-acetyl-2,5-dimethylfuran in food.
关键词
气相色谱-质谱法/3-乙酰基-2,5-二甲基呋喃/食品/测定
Key words
gas chromatography-mass spectrometry/3-acetyl-2,5-dimethylfuran/food/determination
维普
万方数据