A method for simultaneous determination of 21 food additives in several food matrices by ultra high performance liquid chromatography-triple quadrupole tandem mass spectrometry was established.Using pure water and acetonitrile-methanol-water solution(V∶V∶V,1∶1∶1)for oscillation and ultrasonic to extracted target compounds in the samples.Ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry was used for analysis and detection in negative ion mode under multi-reaction monitoring(MRM).The separation and analysis were performed on a Waters Acquity UPLC HSS T3(2.1 mm×100 mm,1.8 μm),gradient elution,quantitative with external standard method.The results showed that within the range of 4.9-771.2 ng/mL,each target compound had a good linear relationship,and all the correlation coefficient were greater than or equal to 0.9903.The detection limit of the method were 0.04-0.14 mg/kg,and the quantitative limit of the method were 0.10-0.40 mg/kg.The average recoveries of 21 food additives were 69.2％-118.0％when spiked into blank samples at concentrations of 2,5,10 times the limit of quantification(LOQ)concentration,the relative standard deviation were 0.59％-10.15％,and the instrument precision relative standard deviation(RSD)were 0.68％-4.61％.The established method is simple and fast,and can be widely applied to the analysis and detected various additives in different food matrices.
关键词
添加剂/食品基质/超高效液相色谱-三重四级杆串联质谱/检测
Key words
additives/food matrices/ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry/detection
维普
万方数据