In order to improve the yield of Laetiporus sulphureus polysaccharide(LSP)and analyze the structure and antioxidant activity of LSP,LSP was extracted by ultrasound-assisted complex enzymatic method.The extraction conditions were optimized by single factor and response surface method.High performance liquid chromatography(HPLC),Fourier infrared spectroscopy(FT-IR)and Raman spectroscopy(Ram)were used to analyze the structural characteristics and the antioxidant activity was evaluated by measuring the iron ion reducing ability and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)radical cation,1,1-diphenyl-2-picrylhydrazyl(DPPH)radical,hydroxyl free radical scavenging ability.The extraction process was optimized as ultrasonic time 30.719 min,enzymatic time 79.017 min,solid-liquid ratio 1∶30.634.Under these conditions,the LSP yield was(4.36±0.036)％.LSP is an α-pyranose polysaccharide composed of mannose,glucose,galactose and fucose,and the molar ratio was 22.03∶42.26∶28.25∶7.46.The molecular weight distribution was inhomogeneity.It exhibited antioxidant effect in vitro.The study provided data support for the use of Laetiporus sulphureus polysaccharide as potential natural antioxidants.
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