Ultrasonic-Assisted Composite Enzyme Extraction and Antioxidant Activity of Laetiporus sulphureus Polysaccharide
In order to improve the yield of Laetiporus sulphureus polysaccharide(LSP)and analyze the structure and antioxidant activity of LSP,LSP was extracted by ultrasound-assisted complex enzymatic method.The extraction conditions were optimized by single factor and response surface method.High performance liquid chromatography(HPLC),Fourier infrared spectroscopy(FT-IR)and Raman spectroscopy(Ram)were used to analyze the structural characteristics and the antioxidant activity was evaluated by measuring the iron ion reducing ability and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)radical cation,1,1-diphenyl-2-picrylhydrazyl(DPPH)radical,hydroxyl free radical scavenging ability.The extraction process was optimized as ultrasonic time 30.719 min,enzymatic time 79.017 min,solid-liquid ratio 1∶30.634.Under these conditions,the LSP yield was(4.36±0.036)％.LSP is an α-pyranose polysaccharide composed of mannose,glucose,galactose and fucose,and the molar ratio was 22.03∶42.26∶28.25∶7.46.The molecular weight distribution was inhomogeneity.It exhibited antioxidant effect in vitro.The study provided data support for the use of Laetiporus sulphureus polysaccharide as potential natural antioxidants.
万方数据
维普
