摘要
目的:探究蓝莓花色苷对小鼠运动疲劳的影响.方法:采用灌胃方式喂食不同剂量蓝莓花色苷,以蒸馏水作为阴性对照,牛磺酸作为阳性对照,通过力竭跑步试验评价抗疲劳活性,同时测定疲劳相关指标.结果:提取蓝莓花色苷质量浓度为(432.15±9.71)mg/g,和阴性对照组相比,不同剂量组和牛磺酸组对小鼠体质量和器官指数无显著影响(P＞0.05);高剂量组蓝莓花色苷显著延长了小鼠力竭跑步时间(P＜0.05),肌酸激酶(Creatine kinase,CK)、血清乳酸(Blood lactic acid,BLA)、血清尿素氮(Blook urea nitrogen,BUN)和丙二醛(Malondialdehyde,MDA)水平最低(P＜0.05),肝糖原、肌糖原、乳酸脱氢酶(Lactate dehydrogenase,LDH)、超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)水平最高(P＜0.05),与牛磺酸组效果相当.结论:研究表明蓝莓花色苷能改善小鼠运动疲劳,可为功能性食品的开发提供原料.
Abstract
Objective:To explore the effect of blueberry anthocyanins on exercise fatigue in mice.Methods:Different doses of blueberry anthocyanins were fed by gavage,distilled water was used as negative control and taurine was used as positive control.The anti-fatigue activity was evaluated by exhaustion running test,and fatigue related indexes were determined.Results:The concentration of blueberry anthocyanins was(432.15±9.71)mg/g.Compared with the negative control group,different dosage groups and taurine group had no significant effects on body weight and organ index of mice(P＜0.05).High-dose blueberry anthocyanins significantly extended the exhausted running time of mice(P＜0.05),creatine kinase(CK),blood lactic acid(BLA),blook urea nitrogen(BUN)and malondialdehyde(MDA)levels were the lowest(P＜0.05),liver glycogen,muscle glycogen,lactate dehydrogenase(LDH),superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)levels were the highest(P＜0.05),the effect was similar to that of taurine group.Conclusion:The results suggest that blueberry anthocyanins could improve the exercise fatigue of mice and provide raw materials for the development of functional food.
维普
万方数据