Determination of Acaroquinone and Its Metabolites in Tea by Modified SinChERS Combined with Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
A detection method based on SinChERS purification technology combined with ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was established to determine the residual levels of acaricide quinone and its metabolite hydroxy acaricide quinone in tea.The sample was extracted by 1％formic acid acetonitrile,purified by SinChERS-TPR,separated by Agilent C18 chromatographic column,gradient eluted with 0.1％formic acid water and methanol as mobile phase,detected by electrospray ion(ESI)source,positive ion mode,multiple reaction monitoring(MRM)mode,and quantified by matrix matching working curve external standard method.The linear relationship between acaroquinone and hydroxy acaroquinone exhibited good linearity in the range of 0.200 ng/mL to 20.0 ng/mL,with correlation coefficients greater than 0.999.The limit of detection for acariquinone was 1.25μg/kg,and for hydroxy acaroquinone was limited to 0.50 μg/kg;the limit of quantitation for acaroquinone was 4 μg/kg,and for the hydroxy acaroquinone was 2 pg/kg.The recovery rates of the three levels of acaroquinone and hydroxy acaroquinone were 77.0％to 100.2％,with a relative standard deviation of 1.7％to 7.8％.This method was easy to operate,fast and accurate,and was suitable for batch determination of acaroquinone and its metabolites in tea.
万方数据
维普
