Determination of Bongkrekic Acid in Guangxi Fresh Rice Noodles by Ultra Performance Liquid Chromatograghy-Tandem Mass Spectrometry
Objective:A method for the determination of bongkrekic acid in fresh rice noodles of Guangxi Province was established by using mixed-mode anion exchange solid phase extraction combined with ultra performance liquid chromatograghy-tandem mass spectrometry(UPLC-MS).And the risk of fresh rice noodles contaminated by bongkrekic acid was preliminarily assessed.Methods:Fresh rice noodles of Guangxi Province were taken as the research objects.Mixed-mode anion exchange columns were applied to purify the target compound.The 0.1％formic acid water and acetonitrile were used as mobile phase for gradient elution.Electrospray ion source negative mode and external standard method were applied to detect the samples.Results:Bongkrekic acid showed good linear relationship in the concentration range of 1.00-50.00 ng/mL,and the correlation coefficient was 0.99996.Via the optimized solid phase extraction,the limit of detection was 0.03 μg/kg,and the limit of quantification was 0.1 μg/kg.Three adding levels(low,medium,high)were carried out,and the recovery rate,were 97.0％-102.1％,and the relative standard deviations were 1.4％-2.7％.Conclusion:The method showed high sensibility and good accuracy,which could meet the needs of the determination of bongkrekic acid in Guangxi fresh rice noodles.The results of sampling tests indicated that the risk of fresh rice noodles contaminated by bongkrekic acid was low.
万方数据
维普
