Effect of Lonicera caerulea Berry Polyphenols on Regulate Intestinal Health and Relief Exercise Fatigue of Mice in Low Temperature
Long-term high-intensity sports would cause fatigue damage and reduce the performance of athletes,especially low-temperature sports.This study evaluated the effects of Lonicera caerulea berry polyphenols(LCBP)on treadmill fatigue in mice at low temperature(-5 ℃).With VC as positive control,colon oxidation,product metabolism(lactic acid(LA),blood urea nitrogen(BUN)),hemoglobin(Hb),creatine kinase(CK),lactate dehydrogenase(LDH)and other biochemical parameters were determined.Colon pathology was detected by hematoxylin-eosin(H&E)staining.The short-chain fatty acid targeted metabolism of colon contents was detected by gas chromatography-tandem mass spectrometry(GC-MS),and intestinal flora was detected by 16S rRNA high-throughput sequencing.The results showed that compared with model group,dietary supplementation with LCBP significantly extended the exhaustion time of treadmill exercise by 27.4％(P＜0.01).Hb levels in blood were increased,and LA,BUN and CK levels were significantly decreased(P＜0.01).Dietary supplementation with LCBP(250 mg/kg bw)decreased the malondialdehyde(MDA)level in colonic tissues induced by low temperature exercise significantly(P＜0.01),meanwhile,increased the activity of antioxidant enzymes,and the total antioxidant level(P＜0.01).LCBP promoted the formation of acetic acid,propionic acid,butyric acid,valeric acid and caproic acid in short-chain fatty acids(P＜0.01),improved intestinal flora significantly.And increased the abundance and species of beneficial bacteria such as Prevotella,Lachnospira and Bifidobacterium which promoted intestinal health.In conclusion,dietary supplementation of LCBP reduce oxidative stress,metabolite accumulation,balance intestinal flora homeostasis and relieve fatigue during low-temperature exercise in mice.
万方数据
维普
