Physicochemical Properties and Antioxidant Activity of Graded Alcohol Precipitation of Polysaccharides from Baccaurea ramiflora Lour Peel
Crude polysaccharides of Baccaurea ramiflora Lour peel was extracted by hot water method,and six polysaccharides fractions,PBP-30,PBP-40,PBP-50,PBP-60,PBP-70,PBP-80,were obtained by ethanol graded alcoholic precipitation method.The physicochemical properties and antioxidant activities of the six polysaccharides fractions were analyzed comparatively.The results showed that PBP-30 had the highest yield,total sugar content,glucuronic acid content,and protein content of(12.92±0.44)％,(77.13±1.53)％,(7.14±0.26)％,and(4.89±0.92)％,respectively,which were significantly different compared to the other fractions(P＜0.05).The hydroxyl radical scavenging ability,1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging ability,superoxide anion radical scavenging ability,2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)cation radical scavenging ability and reducing power of the six polysaccharides fractions were gradually enhanced with the increase of concentration.The results demonstrated that each polysaccharides fraction of Baccaurea ramiflora Lour peel has good antioxidant activity and is expected to be developed as a novel natural antioxidant.
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