食品科技2024,Vol.49Issue(8) :192-199.

分级醇沉木奶果皮多糖的理化性质及抗氧化活性研究

Physicochemical Properties and Antioxidant Activity of Graded Alcohol Precipitation of Polysaccharides from Baccaurea ramiflora Lour Peel

郑婷婷 李兵 龚婉莹 赵苗苗 刘聪 王礼中 张文杰 严亮
食品科技2024,Vol.49Issue(8) :192-199.

分级醇沉木奶果皮多糖的理化性质及抗氧化活性研究

Physicochemical Properties and Antioxidant Activity of Graded Alcohol Precipitation of Polysaccharides from Baccaurea ramiflora Lour Peel

郑婷婷 1李兵 2龚婉莹 3赵苗苗 3刘聪 3王礼中 4张文杰 3严亮3
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作者信息

  • 1. 滇西应用技术大学普洱茶学院,云南普洱 665000;普洱茶研究院,云南普洱 665000;云南农业大学食品科学技术学院,云南 昆明 650201
  • 2. 滇西应用技术大学普洱茶学院,云南普洱 665000
  • 3. 滇西应用技术大学普洱茶学院,云南普洱 665000;普洱茶研究院,云南普洱 665000
  • 4. 澜沧良宝生物科技有限公司,云南澜沧 665699
  • 折叠

摘要

以木奶果皮为原料,采用热水法提取木奶果皮粗多糖,通过乙醇分级醇沉法制备得到6个多糖组分(PBP-30、PBP-40、PBP-50、PBP-60、PBP-70、PBP-80),对比分析6个多糖组分的理化性质及抗氧化活性.结果表明,PBP-30的得率、总糖含量、糖醛酸含量、蛋白质含量最高,分别为(12.92±0.44)%、(77.13±1.53)%、(7.14±0.26)%、(4.89±0.92)%,相比其他组分存在显著性差异(P<0.05).6种多糖组分对羟自由基、1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl,DPPH)自由基、超氧阴离子自由基、2,2-联氨-双-3-乙基苯并噻唑啉-6-磺酸(2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基的清除能力及还原力均随着浓度的增加逐渐增强,表明木奶果皮各多糖组分具有良好的抗氧化活性,有望开发成为新型天然抗氧化剂.

Abstract

Crude polysaccharides of Baccaurea ramiflora Lour peel was extracted by hot water method,and six polysaccharides fractions,PBP-30,PBP-40,PBP-50,PBP-60,PBP-70,PBP-80,were obtained by ethanol graded alcoholic precipitation method.The physicochemical properties and antioxidant activities of the six polysaccharides fractions were analyzed comparatively.The results showed that PBP-30 had the highest yield,total sugar content,glucuronic acid content,and protein content of(12.92±0.44)%,(77.13±1.53)%,(7.14±0.26)%,and(4.89±0.92)%,respectively,which were significantly different compared to the other fractions(P<0.05).The hydroxyl radical scavenging ability,1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging ability,superoxide anion radical scavenging ability,2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)cation radical scavenging ability and reducing power of the six polysaccharides fractions were gradually enhanced with the increase of concentration.The results demonstrated that each polysaccharides fraction of Baccaurea ramiflora Lour peel has good antioxidant activity and is expected to be developed as a novel natural antioxidant.

关键词

木奶果皮/多糖/分级醇沉/理化性质/抗氧化活性

Key words

Baccaurea ramiflora Lour peel/polysaccharides/graded alcohol precipitation/physicochemical properties/antioxidant activity

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基金项目

2023年云南省"三区"科技人才项目()

2024年云南省科技特派员项目(202304BK090479)

出版年

2024
食品科技
北京市粮食科学研究所

食品科技

CSTPCD北大核心
影响因子:0.622
ISSN:1005-9989
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