In order to improve flavonoids content and assess functional activity of flavonoids extract from Ougan(Citrus Suavissima Hort.Ex Tanak)peel,the ultrasound assisted ethanol extraction and macroporous resin purification process of flavonoids from freeze-dried Ougan peel were optimized,and their functional activities were comparatively evaluated.Results showed that the highest flavonoid content(118.94±2.29)mg/g,in the extract was obtained under the conditions of 40％ethanol,30∶1(mL/g)in liquid to material ratio,and 400 W,60 ℃ and 60 min for ultrasound power,temperature and time,respectively.The optimal purification process for AB-8 macroporous resin was as follows:sample concentration,sample flow rate,elution solvent,and elution flow rate were 3.5 mg/mL,0.75 mL/min,60％ethanol,and 0.75 mL/min,respectively.The purity of flavonoid in the extract after purification was enhanced around 3.07 folds.The flavonoids extract mainly contained neohesperidin,nobiletin,hesperidin,tangeretin,poncirin,naringin and 5-demethylnobiletin,and their respective content was increased and the total amount was enhanced in 2.63 folds after purification.The purified flavonoids extract exhibited higher activities in scavenging 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)cation radical,1,1-diphenyl-2-picrylhydrazyl(DPPH)radical and hydroxyl radicals,and ferric ion reducing antioxidant power(FRAP),as well as inhibitory activity on α-glucosidase.The study indicated that flavonoids extract from Ougan peel had high antioxidative and in vitro hypoglycemic activities with higher abilities after purification.
维普
万方数据
