Extraction and Purification Processes of Flavonoids from Ougan(Citrus suavissima Hort.Ex Tanak)Peel and Its Biological Activity Evaluation
In order to improve flavonoids content and assess functional activity of flavonoids extract from Ougan(Citrus Suavissima Hort.Ex Tanak)peel,the ultrasound assisted ethanol extraction and macroporous resin purification process of flavonoids from freeze-dried Ougan peel were optimized,and their functional activities were comparatively evaluated.Results showed that the highest flavonoid content(118.94±2.29)mg/g,in the extract was obtained under the conditions of 40％ethanol,30∶1(mL/g)in liquid to material ratio,and 400 W,60 ℃ and 60 min for ultrasound power,temperature and time,respectively.The optimal purification process for AB-8 macroporous resin was as follows:sample concentration,sample flow rate,elution solvent,and elution flow rate were 3.5 mg/mL,0.75 mL/min,60％ethanol,and 0.75 mL/min,respectively.The purity of flavonoid in the extract after purification was enhanced around 3.07 folds.The flavonoids extract mainly contained neohesperidin,nobiletin,hesperidin,tangeretin,poncirin,naringin and 5-demethylnobiletin,and their respective content was increased and the total amount was enhanced in 2.63 folds after purification.The purified flavonoids extract exhibited higher activities in scavenging 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)cation radical,1,1-diphenyl-2-picrylhydrazyl(DPPH)radical and hydroxyl radicals,and ferric ion reducing antioxidant power(FRAP),as well as inhibitory activity on α-glucosidase.The study indicated that flavonoids extract from Ougan peel had high antioxidative and in vitro hypoglycemic activities with higher abilities after purification.
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