Optimization of Ultrasonic Extraction of Flavonoids from Chenopodium quinoa after Germination by Plackett-Burman and Box-Behnken Experiment and Its Hypoglycemic Activity in Vitro
Objective:The conditions of ultrasonic extraction of flavonoids from Chenopodium quinoa after germination was optimized and the hypoglycemic activity was studied in vitro.Method:Significant factors in the extraction including ratio of solid to liquid,ultrasonic power,ultrasonic time,ethanol content,and extraction temperature,extraction times were determined by Plackett-Burman design,then the steepest ascent method and Box-Behnken experiment were used to optimize the extraction conditions.The in vitro hypoglycemic activity of extraction was studied based on their inhibitory effects on α-amylase andα-glucosidase.Result:The significant factors selected by Plackett-Burman test were ratio of solid to liquid,ultrasonic power,ethanol content,and extraction temperature,and according to response surface methodology,the optimum extraction conditions of ratio of solid to liquid 1∶26(g/mL),ultrasonic power 120 W,ethanol content 55％,and extraction temperature 60 ℃,the extraction yield of Chenopodium quinoa flavonoids after germination was(3.07±0.13)mg/g.The inhibition activities of flavonoids from Chenopodium quinoa after germination to α-amylase and α-glucosidase were better than Chenopodium quinoa flavonoids with IC50=11.34 µg/mL,8.79 µg/mL,respectively.Conclusion:The extraction process of flavonoids from Chenopodium quinoa after germination was stable,and the flavonoids had strong hypoglycemic activity in vitro.
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