Determination of Different Selenium Speciation in 5 Kinds of Cruciferous Plants by HPLC-ICP-MS
Objective:To establish a high performance liquid chromatography-inductively coupled plasma-mass spectrometry(HPLC-ICP-MS)method in the determination of five selenium species,including selenocystine(SeCys2),methylselenocysteine(MeSeCys),tetravalent selenium(Se(Ⅳ)),selenomethionine(SeMet),and hexavalent selenium(Se(Ⅵ))in cruciferous plants and to investigate the enrichment capacities of these selenium species in five different cruciferous plants.Methods:The samples were extracted with ultrasound-assisted protein E and cellulase.Separation was performed using a Hamilton PRP-X100 column with(NH4)2HPO4 as the mobile phase.The separation of five selenium species in Cardamine violifolia,cabbage,broccoli,oilseed rape,and radish leaves was achieved by HPLC,and quantification was conducted using ICP-MS.Results:Different selenium speciation was separated in 18 min,showed a good linear relationship between 0 to 200 μg/L,correlation coefficients were both greater than 0.999.Detection(LOD)of SeCys2,MeSeCys,Se(Ⅳ),SeMet,Se(Ⅵ)were 0.005,0.025,0.005,0.010,0.025 mg/kg,with relative standard deviations(RSD)of 2.4％,3.1％,1.6％,3.3％,and 2.1％.Recovery rates were within the acceptable range from 79.3％to 103.5％.Significant differences were found in selenium species and content among the five cruciferous plants.In cabbage,SeCys2,SeMet,and Se(Ⅵ)had higher contents,while MeSeCys showed relatively low detection.In Cardamine violifolia,SeCys2 and SeMet contents were higher.All five selenium species were detected in radish leaves,but their contents were relatively low,with SeCys2 being slightly higher.In broccoli,SeCys2,SeMet,and Se(Ⅵ)were detected,with SeMet and Se(Ⅵ)having the highest contents.In oilseed rape,SeCys2 and SeMet were mainly detected,with higher contents.Conclusion:The established method is suitable for determining different selenium species in cruciferous plants,offering simplicity,a wide linear range,and high sensitivity.Cruciferous plants show a strong ability to accumulate selenium,with higher detection rates of SeCys2,SeMet,and Se(Ⅵ).However,the enrichment ability of different cruciferous plants for different selenium species varies significantly.
万方数据
维普
