Determination of Validamycin A residues in Baby Rice Flour
An analytical method was developed for the determination of validamycin A in baby rice flour by hydrophilic interaction liquid chromatography coupled with tandem-mass spectrometry.The target compound was extracted with methanol-water(7∶3),1 mL of the extract solution was evaporated to dry under nitrogen flow,then redissolved in 20 mmol/L ammonium acetate(pH3.0)and cleaned-up with MCX solid phase extraction column.The separation was carried out on a BEH Amide column using 5 mmol/L ammonium acetate-acetonitrile as mobile phase and detection was performed using electrospray ionization mass spectrometry in multiple reaction monitoring mode,with quantification done using the external standard method.The method showed good linearity(R2＞0.99)within the range of 0.1 ng/mL to 5.0 ng/mL.The limits of detection and quantification were 2.0 pg/kg and 5.0 pg/kg,respectively.Recovery experiments spiked at levels of 5.0,50.0,200.0 μg/kg in blank baby rice flour yielded average recoveries of 88.5％to 100.5％,with relative standard deviations of 4.84％to 10.08％.The method is characterized by its simplicity in extraction and purification,good reproducibility,and high sensitivity,making it suitable for the rapid qualitative and quantitative analysis of validamycin A in baby rice flour.
万方数据
维普
