Qualitative Detection Methods of Animal Derived Ingredients in Duck Blood by Real-Time Fluorescence PCR
Objective:In order to detect the adulteration of animal derived ingredients in duck blood products,a qualitative detection method was developed in this paper for real-time fluorescence polymerase chain reaction(PCR)of animal derived components such as chicken,duck,goose,pig,cow,and sheep in duck blood.Method:The method investigated the impact of three different DNA extraction methods on the results,explored the detection limit of the method,and tested 51 duck blood samples.Results:The DNA concentration and amplification efficiency extracted by drying and weighing were the highest,and the sensitivity of the method could reach 1％.In actual samples detection,8 out of 51 duck samples were found to have other animal derived ingredients.Conclusion:This method realize the qualitative detection of other animal derived components in duck blood,providing strong technical support for law enforcement and regulatory authorities.
万方数据
维普
